Box-Behnken Optimized One-Step CO-Synthesis of Poly(pyrocatechol violet)-Reduced Graphene
Researchers at Vietnamese universities fabricated a glassy carbon electrode modified with poly(pyrocatechol violet) and electrochemically reduced graphene oxide using one-step cyclic voltammetry, achieving a metronidazole detection limit of 0.013 µM. The work demonstrates a route to functionalized reduced graphene oxide composites that avoids toxic chemical reductants, relevant to the development of graphene-based electrochemical sensing materials.
Researchers have developed an advanced electrochemical sensor for the detection of Metronidazole, utilizing a nanocomposite of poly(pyrocatechol violet) and electrochemically reduced graphene oxide. This sensor was fabricated by modifying a glassy carbon electrode through a one-step cyclic voltammetry process. This method facilitated the simultaneous electropolymerization of the monomer and reduction of graphene oxide, eliminating the need for toxic chemical reducing agents. The synthesis conditions were optimized using a Box-Behnken Design to enhance the electrochemical response.
The modified glassy carbon electrode demonstrated superior electrocatalytic activity for Metronidazole reduction, showing a significant increase in peak current compared to bare and single-component-modified electrodes. Under optimal conditions, the sensor provided a wide linear working range from 0.1 to 20 µM and a low detection limit of 0.013 µM. The method's precision was validated with relative standard deviations under 5.1% for repeatability and 3.7% for reproducibility. Additionally, the sensor exhibited high selectivity against common biological interferents and inorganic ions.
The practical application of this method was confirmed by successfully determining Metronidazole in human urine samples. The results were statistically comparable to those obtained using High-Performance Liquid Chromatography. This study highlights the potential of poly(pyrocatechol violet)/reduced graphene oxide composites in developing sensitive and selective electrochemical sensors.
Source: Graphene Feed